fgf10  (Miltenyi Biotec)

Journal: Biomaterials Research

Article Title: Photocrosslinkable Chitosan Quaternary Ammonium Salt-Based Ternary Hydrogel with Fibroblast Growth Factor 21 for Diabetic Wound Healing

doi: 10.34133/bmr.0309

Figure Lengend Snippet: Schematic diagram of the development of G/C-CS@FGF-21 hydrogel for diabatic wound healing. GelMA, gelatin methacrylate; Col, type I collagen; CQS, chitosan quaternary ammonium salt; CQS-MA, methacrylated CQS; FGF-21, fibroblast growth factor 21; UV, ultraviolet; EGF, epidermal growth factor; KGF, keratinocyte growth factor; VEGF-A, vascular endothelial growth factor A; IL-6, interleukin-6; TNF-α, tumor necrosis factor-α; iNOS, inducible nitric oxide synthase; ARG-1, arginase 1.

Article Snippet: Recombinant human FGF-21 (HY-P717) was sourced from MedChemExpress (Monmouth Junction, NJ, USA).

Techniques:

Journal: Biomaterials Research

Article Title: Photocrosslinkable Chitosan Quaternary Ammonium Salt-Based Ternary Hydrogel with Fibroblast Growth Factor 21 for Diabetic Wound Healing

doi: 10.34133/bmr.0309

Figure Lengend Snippet: Biological analysis of RAW264.7 and L929 cells stimulated with various concentrations of FGF-21. (A to D) qRT-PCR of M1- and M2-related gene expressions in RAW264.7 cells. (E) Immunofluorescence staining of M1- and M2-related proteins in RAW264.7 cells. (F and G) Western blotting and quantification analysis of M1- and M2-related protein expressions in RAW264.7 cells. (H and I) Scratch assay and quantification analysis of L929 cells stimulated with various concentrations of FGF-21. Data are presented as mean ± SD ( n = 3). * P < 0.05, ** P < 0.01, and *** P < 0.001 vs. G group; ns, not significant. MCP-1, monocyte chemoattractant protein-1; DAPI, 4′,6-diamidino-2-phenylindole.

Article Snippet: Recombinant human FGF-21 (HY-P717) was sourced from MedChemExpress (Monmouth Junction, NJ, USA).

Techniques: Quantitative RT-PCR, Immunofluorescence, Staining, Western Blot, Wound Healing Assay

Journal: Biomaterials Research

Article Title: Photocrosslinkable Chitosan Quaternary Ammonium Salt-Based Ternary Hydrogel with Fibroblast Growth Factor 21 for Diabetic Wound Healing

doi: 10.34133/bmr.0309

Figure Lengend Snippet: Transcriptional profiles for RAW264.7 cells on hydrogels. (A) Statistics of differently expressed genes between all 3 compositions of hydrogels. (B) Volcano plot transcriptional landscape between G/C and G/C-CS@FGF-21. The x -axis represents to the log 2 -transformed fold change, while the y -axis corresponds to the negative logarithm of the P value following adjustment for multiple comparisons. (C) The heat map of wound-healing- and inflammation-related genes between G/C and G/C-CS@FGF-21. (D) Kyoto Encyclopedia of Genes and Genomes (KEGG) enrichment analysis between G/C and G/C-CS@FGF-21. (E) Gene Ontology (GO) enrichment analysis between G/C and G/C-CS@FGF-21. (F) Gene set enrichment analysis (GSEA) between G/C and G/C-CS@FGF-21. (G) Protein–protein interaction networks of differentially expressed proteins between G/C and G/C-CS@FGF-21.

Article Snippet: Recombinant human FGF-21 (HY-P717) was sourced from MedChemExpress (Monmouth Junction, NJ, USA).

Techniques: Transformation Assay

Journal: Biomaterials Research

Article Title: Photocrosslinkable Chitosan Quaternary Ammonium Salt-Based Ternary Hydrogel with Fibroblast Growth Factor 21 for Diabetic Wound Healing

doi: 10.34133/bmr.0309

Figure Lengend Snippet: In vivo evaluation of FGF-21-loaded hydrogel in a full-thickness wound-healing model. (A) Schematic diagram of the experimental timeline and treatment protocol for wound healing in a diabetic mouse model. (B) Representative photographic documentation and diagrammatic overview of wound closure from days 0 to 15. (C) Quantified wound closure rates over the 15-d observation period. (D) Histological analysis via hematoxylin and eosin (H&E) staining on days 12 and 15. (E) Collagen deposition assessed by Masson’s trichrome staining on days 12 and 15. Morphometric evaluation of (F) wound width, (G) hair follicle density, and (H) granulation tissue thickness. Data are presented as mean ± SD ( n = 3). * P < 0.05, ** P < 0.01, and *** P < 0.001 vs. G group; ns, not significant. IHC, immunohistochemistry; WB, Western blotting.

Article Snippet: Recombinant human FGF-21 (HY-P717) was sourced from MedChemExpress (Monmouth Junction, NJ, USA).

Techniques: In Vivo, Staining, Immunohistochemistry, Western Blot